4. DNA Extraction and Quality Control
DNA Extraction and Quality Control
Rachel Watt, Thomas Atkinson, Sarah Phillips, Steve James and Evelien Adriaensesens
Here we discuss the DNA extraction methods used at the Quadram Institute to evaluate the microbiome from various samples collected as part of human microbiome studies. The methods for quality control and DNA/RNA enumeration of extracted DNA and its importance for sequencing and downstream analysis are also explained.
4. DNA Extraction and Quality Control v1.0 [pdf]
Quadram Institute Best Practice in Microbiome Research: DNA Extraction and Quality Control v1.0 by Rachel Watt, Thomas Atkinson, Sarah Phillips, Steve James and Evelien Adriaenssens is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
- Introduction
- 1. Study Design Considerations
- 2. Sample Size for a Microbiome Experiment
- 3. Collection & Processing of Faecal Samples
- 4. DNA Extraction and Quality Control
- 5. 16S Illumina Library Preparation
- 6. Shotgun Metagenomic Illumina Library Preparation
- 7. Microbiome Informatics
- 8. Statistical Analysis of Microbiome Data
- 9. Viromics-Specific Protocols
- 10. Mycobiome-Specific Protocols
- About the Quadram Institute