6. Shotgun Metagenomic Illumina Library Preparation

Shotgun Metagenomic Illumina Library Preparation

Dave Baker

Here we describe how to take metagenomic DNA and process it into a viable Illumina library for DNA sequencing. One way of determining the species or genera in complex metagenomics samples is using Shotgun Whole Genome Sequencing (WGS). Up until recently this was unaffordable and so the cheaper targeted 16S approached was preferred. With the advent of Next Generation Sequencing and the improvement of these technologies over time, the cost of sequencing has significantly reduced (as low as £100 per sample). Projects that need to assemble novel unculturable bacterial species, and where higher resolution is required, will benefit from this approach [1].

6. Shotgun Metagenomic Illumina Library Preparation v1.0[pdf]

Quadram Institute Best Practice in Microbiome Research: Shotgun Metagenomic Illumina Library Preparation v1.0 by David Baker is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.