10. Mycobiome-Specific Protocols

Mycobiome-Specific Protocols

Steve James

The following protocols are a set of methods used at the Quadram Institute (QI) to study and characterise the fungal community that resides within the human gastrointestinal (GI) tract, namely the gut mycobiome. The culture-based methods have been developed to isolate and culture, where possible, viable enteric fungi from fresh or refrigerated human faecal samples. The non-culture-based protocols provide a workflow for isolating fungal DNA from human faecal samples that can be used as a template to prepare amplicon libraries for fungal community profiling using the Illumina MiSeq sequencing platform.

10. Mycobiome-Specific Protocols v1.0 [pdf]

Quadram Institute Best Practice in Microbiome Research: Mycobiome-Specific Protocols v1.0 by Steve James is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.