My first contact with research was while working in the Venezuelan Amazon with Amerindian populations and diagnosis/epidemiology of tuberculosis. During my master I worked on the dynamics of the Pseudomonas aeruginosa biofilm associated with prostheses and surgical surfaces. Then for my PhD, I worked on Mycobacterium abscessus, focusing on two main areas: Molecular mechanisms of drug resistance and cell wall biosynthesis and their impact on virulence using zebrafish as a model of infection. I am currently working on the functional genomics of Listeria to stress associated with the food chain.
Gutiérrez, A. V., Richard, M., Roquet-Banères, F., Viljoen, A., & Kremer, L. (2019). The TetR family transcription factor MAB_2299c regulates the expression of two distinct MmpS-MmpL efflux pumps involved in cross-resistance to clofazimine and bedaquiline in Mycobacterium abscessus. Antimicrobial agents and chemotherapy, 63(10), e01000-19. https://doi.org/10.1128/AAC.01000-19.
Richard, M., Gutiérrez, A. V., Viljoen, A., Rodriguez-Rincon, D., Roquet-Baneres, F., Blaise, M., & Kremer, L. (2019). Mutations in the MAB_2299c TetR regulator confer cross-resistance to clofazimine and bedaquiline in Mycobacterium abscessus. Antimicrobial agents and chemotherapy, 63(1). https://doi.org/10.1128/AAC.01316-18.
Richard, M., Gutiérrez, A. V., & Kremer, L. (2020). Dissecting erm (41)-mediated macrolide-inducible resistance in Mycobacterium abscessus. Antimicrobial Agents and Chemotherapy, 64(2). DOI: 10.1128/AAC.01879-19
Viljoen, A., Gutiérrez, A. V., Dupont, C., Ghigo, E., & Kremer, L. (2018). A simple and rapid gene disruption strategy in Mycobacterium abscessus: on the design and application of glycopeptidolipid mutants. Frontiers in cellular and infection microbiology, 8, 69. DOI: 10.3389/fcimb.2018.00069
Richard, M., Gutiérrez, A. V., Viljoen, A. J., Ghigo, E., Blaise, M., & Kremer, L. (2018). Mechanistic and structural insights into the unique TetR-dependent regulation of a drug efflux pump in Mycobacterium abscessus. Frontiers in microbiology, 9, 649. DOI: 10.3389/fmicb.2018.00649