Bruce Pearson

Laboratory Manager

Contact via email

Laboratory Manager for floors 3 and 4 (Microbiology and Molecular Biology). Former Deputy Head of Campylobacter research at the Quadram Institute Bioscience. Research activity: Genome sequencing of Campylobacter coli. Campylobacter microarrays focusing on gene functional analysis relating to metabolism in relation to small RNA, plasmids and environmental stress responses including external collaborations. Chaired Health and Safety committee. Chaired Institute working groups on Communications, and Health and Wellbeing. Deputy Institute Fire Officer.

Molecular Microbiology, DNA sequencing, Microarrays, Campylobacter, Yeast, Bacterial Genetics, Genome assembly.

I am a senior molecular microbiologist. I started my career studying biochemistry at the University of Edinburgh before moving to the Institute in Norwich at the start up of a yeast group to investigate yeast functional genomics. I was involved in the successful yeast genome-sequencing project: sequencing part of chromosome iii; the first eukaryotic chromosome to be completely sequenced and then sequencing two large regions of chromosome xv to help complete the entire yeast genome. During subsequent EU-funded functional genomics initiatives, eurofan and eurofan ii, I completed two “sixpacks” and developed a number of approaches for gene disruption in yeast. I also created a PCR-based method for dna fingerprinting yeast, which was patented and is currently the method of choice for strain discrimination used commercially by the National Collection of Yeast Cultures. With over 30 years in molecular biology, I was central to initiating Campylobacter microarrays at IFR and influential in the strategic development of a dual approach using 2-d proteomics and DNA microarrays to analyse Campylobacter stress responses. I was a Grant Holder and PI on a successful BBSRC research project:  ‘host acute stress responses and the regulation of C. jejuni virulence in the avian gut, in collaboration with Professor Julian Ketley and Professor Tom Humphrey .

The project investigated the influence on Campylobacter of stress hormones released into the gut lumen. Recently, I have been a key part of the team, that, in collaboration with intervet/schering-plough animal health were able to completely sequence and annotate the genome of Campylobacter jejuni 81116 and has gone on to sequence approximately 500 Campylobacter coli strains. Research interests centre around linking genotype and phenotype through functional genomics. I was acting Head of Campylobacter research between November 2005 and March 2007 and with 35 years experience am currently laboratory manager for floors 3 and 4.

Key Publications

Pearson B. M., Louwen R. P., van Baarlen P., van Vliet A. H. M.(2015) Differential distribution of Type II CRISPR-Cas systems in agricultural and non-agricultural Campylobacter coli and Campylobacter jejuni isolates correlates with lack of shared environments. Genome Biology and Evolution  7 2663-2679.

Hanfrey C., Pearson B. M., Gaskin D. J. H., Lee J., Woster P. M., Phillips M., Michael A. J.(2011) An alternative spermidine biosynthetic route is critical for growth of Campylobacter jejuni and is the dominant polyamine pathway in the human gut microbiota Journal of Biological Chemistry  286 43301-12.

Thomas M. T., Shepherd M., Poole R. K., van Vliet A. H. M., Kelly D. J., Pearson B. M.(2011) Two respiratory enzyme systems in Campylobacter jejuni NCTC 11168 contribute to growth on L-lactate Environmental Microbiology  13 48-61.

Monk C. E., Pearson B. M., Mulholland F., Smith H. K., Poole R.(2008) Oxygen- and NssR-dependent globin expression and enhanced iron acquisition in the response of Campylobacter to nitrosative stress Journal of Biological Chemistry  283 28413-28425.

Guccione E., Leon-Kemkis M. R., Pearson B. M., Hitchin E., Mulholland F., van Diemen P. M., Stevens M. P., Kelly D. J.(2008) Amino-acid dependent growth of Campylobacter jejuni: Key roles for aspartase (AspA) under microaerobic and oxygen-limited conditions and identification of AspB (Cj0762), essential for growth on glutamate  Molecular Microbiology  69 77-93.

Tejera N,Crossman L,Pearson B,Stoakes E,Nasher F,Djeghout B,Poolman MG,Wain J,Singh D. (2020)

Genome-scale metabolic model driven design of a defined medium for Campylobacter jejuni M1cam

Frontiers in Microbiology, 11, 1072

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Pascoe B., Williams L. K., Calland J. K., Meric G., Hitchings M. D., Dyer M., Ryder J., Shaw S., Lopes B. S., Chintoan-Uta C., Allan E., Vidal A., Fearnley C., Everest P., Pachebat J. A., Cogan T. A., Stevens M. P., Humphrey T. J., Wilkinson T. S., Cody A. J., Colles F. M., Jolley K. A., Maiden M. C. J., Strachan N., Pearson B., Linton D., Wren B. W., Parkhill J., Kelly D. J., van Vliet A. H. M., Forbes K. J., Sheppard S. K.. (2019)

Domestication of Campylobacter jejuni NCTC 11168.

Microbial genomics, 5

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Wood I. P., Pearson B., Garcia Gutierrez E., Havlickova L., He Z., Harper A. L., Bancroft I., Waldron K. W.. (2017)

Carbohydrate microarrays and their use for the identification of molecular markers for plant cell wall composition

Proceedings of the National Academy of Sciences of the United States of America, Early Edition , doi:10.1073/pnas.1619033114

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Le M. T., van Veldhuizen M., Porcelli I., Bongaerts R. J., Gaskin D. J. H., Pearson B. M., van Vliet A. H. M.. (2015)

Conservation of s28-Dependent Non-Coding RNA Paralogs and Predicted s54-Dependent Targets in Thermophilic Campylobacter Species.

PLoS One, 10, e0141627

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Porcelli I., Reuter M., Pearson B. M., Wilhelm T., van Vliet A. H. M.. (2013)

Parallel evolution of genome structure and transcriptional landscape in the Epsilon-proteobacteria

BMC Genomics, 14, 616

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Pearson B. M., Rokney A., Crossman L. C., Miller W. G., Wain J., van Vliet A. H. M.. (2013)

Complete Genome Sequence of the Campylobacter coli Clinical Isolate 15-537360.

Genome Announcements, 1, e01056-13

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Shaw F. L., Mulholland F., Le Gall G., Porcelli I., Hart D., Pearson B. M., van Vliet A. H. M.. (2012)

Selenium-dependent biogenesis of formate dehydrogenase in Campylobacter jejuni is controlled by the fdhTU accessory genes

Journal of Bacteriology, 194, 3814-3823

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