Dr Chloe Bennati-Granier

Researcher

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I am a Post-Doctoral Research scientist in the Diet and Glycobiome Group which is interested in the interaction between bacteria and mucus in the human gastrointestinal tract.

I am currently interested in the proteins the bacteria produces to interact with mucins (heavily glycosylated proteins). My research focuses on the study of CAZymes and the use of those CAZymes in the field of Glycoscience to contribute to new biotechnology applications.

I started to work with Carbohydrate-Active Enzymes (CAZymes) during my Master project, and more especially on the oxidative enzymes active on lignin. I studied the ability of the bacteria Streptomyces for the degradation of micropollutants of wastewater by focusing on laccases enzymes. This work was part of a global project which aims to develop a complementary treatment using constructed wetlands systems and to promote growth of microorganisms able to remove the micropollutants.

During my PhD, I conducted research on studying new fungal CAZymes, the lytic polysaccharide monooxygenases (LPMOs) in the context of second generation bioethanol. The first part of the work was to identify new fungal LPMOs and to express them by using the heterologous system Pichia pastoris. The enzymes were produce small scale and in bioreactor (large scale). The second part was the biochemical characterization of those enzymes. I adapted in vitro enzyme assays, prepared cellulosic substrates, quantified and analysed soluble carbohydrate products by ionic chromatography (DIONEX) and mass spectrometry.

The third part was more applied, by evaluating the contribution of those enzymes in lignocellulosic biomass hydrolysis. Different enzymatic assays on various lignocellulosic biomass allowed to identify the most efficient LPMOs significantly improving the conversion of cellulose by Trichoderma reesei.