Dr Emmanuelle Crost

Researcher

Contact via email

Glycobiology of host-microbe interactions in the gut

I am currently working on the core project entitled “The role of mucus in the cross-talk between gut bacteria and the host”.

I am more especially working on mucin degradation/utilisation by Ruminococcus gnavus, a prominent symbiont of the human gut. We are building up a small collection of R. gnavus strains, all isolated from human faecal samples, and we previously showed that the utilisation of mucins by R. gnavus is strain-dependent. An intramolecular trans-sialidase that plays a role in mucin degradation has been identified in R. gnavus ATCC 29149 and is being characterised in our group.

The goals of this part of the project are: – to understand the differences between the R. gnavus strains by growth assays, genome comparison, transcriptome comparison and enzyme comparison. – to identify and characterise more mucin-degrading enzymes in R. gnavus. – to assess the importance of mucin-degradation in the ability of R. gnavus to colonise and compete in the gut. I am more especially carrying out the growth assays, the genomic/transcriptomic analyses and the in vivo work (personal licence obtained in March 2014).


Key Publications

Crost, E.H., Tailford, L.E., Le Gall, G., Henrissat, B., Fons, M. & Juge, N. (2013) Utilization of host glycans by the human gut symbiont Ruminococcus gnavus is strain dependent. PLOS ONE, 8(10):e76341.

Tailford, L.E., Owen, C.D., Walshaw, J., Crost, E.H., Hardy-Goddard, J., Le Gall, G., de Vos, W.M., Taylor, G.L. & Juge, N. (2015) Discovery of intramolecular trans-sialidases in human gut microbiota suggests novel mechanisms of mucosal adaptation. Nat. Commun., 6:7624.

Crost, E.H., Tailford, L.E., Monestier, M., Swarbreck, D., Henrissat, B., Crossman, L.C. & Juge, N. (2016) The mucin-degradation strategy of Ruminococcus gnavus: The importance of intramolecular trans-sialidases. Gut Microbes, 7(4):302-312.

Wegmann U., Nueno Palop C., Mayer M. J., Crost, E. & Narbad, A. (2017) Complete Genome Sequence of Desulfovibrio piger FI11049, Genome Announcements, 2017 vol. 5 no. 7 e01528-16 Review

Tailford, L.E.*, Crost, E.H.*, Kavanaugh, D* & Juge, N. (2015) Mucin glycan foraging in the human gut microbiome. Front. Genet., 6:81.* Co- first authors

Mukhopadhya I., Morais S., Laverde-Gomez J., Sheridan P. O., Walker A. W., Kelly W., Klieve A. V., Ouwerkerk D., Duncan S. H., Louis P., Koropatkin N., Cockburn D., Kibler R., Cooper P. J., Sandoval C., Crost E., Juge N., Bayer E. A., Flint H. J.. (2018)

Sporulation capability and amylosome conservation among diverse human colonic and rumen isolates of the keystone starch-degrader Ruminococcus bromii.

Environmental Microbiology


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Laverde Gomez J. A., Mukhopadhya I., Duncan S. H., Louis P., Shaw S., Collie-Duguid E., Crost E., Juge N., Flint H. J.. (2018)

Formate cross-feeding and cooperative metabolic interactions revealed by transcriptomics in co-cultures of acetogenic and amylolytic human colonic bacteria.

Environmental Microbiology


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Crost E. H., Le Gall G., Laverde-Gomez J., Mukhopadhya I., Flint H., Juge N.. (2018)

Mechanistic Insights Into the Cross-Feeding of Ruminococcus gnavus and Ruminococcus bromii on Host and Dietary Carbohydrates.

Frontiers in microbiology, 9, 2558


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Wegmann U., Nueno Palop C., Mayer M. J., Crost E. H., Narbad A.. (2017)

Complete Genome Sequence of Desulfovibrio piger FI11049

Genome Announcements, 2017 vol. 5 no. 7 , e01528-16


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Crost E. H., Tailford L. E., Monestier M., Swarbreck D., Henrissat B., Crossman L. C., Juge N.. (2016)

The mucin-degradation strategy of Ruminococcus gnavus: The importance of intramolecular trans-sialidases.

Gut microbes, 7, 302-312


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Tailford L. E., Owen C. D., Walshaw J., Crost E. H., Hardy-Goddard J., Le Gall G., de Vos W. M., Taylor G. L., Juge N.. (2015)

Discovery of intramolecular trans-sialidases in human gut microbiota suggests novel mechanisms of mucosal adaptation.

Nature Communications, 6, 7624


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Crost E., Tailford L., Le Gall G., Fons M., Henrissat B., Juge N.. (2013)

Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent

Plos One, 8, e76341


View Publication

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