People
My interests lie in using comparative genomics, transposon mutagenesis and metabolic reconstruction methods to unpick the impact of genetic mutations upon bacterial niche adaptation.
I completed my undergraduate degree in Biological Sciences at the University of Oxford and went on to study for my PhD at the Wellcome Trust Sanger Institute, where I used both experimental and bioinformatic approaches to investigate the metabolic capacity of closely related salmonellae. At this time, I was part of the team that developed transposon directed insertion-site sequencing (TraDIS), which we used to investigate the essential gene set of Salmonella Typhi grown under laboratory conditions and in the presence of bile. Since then, I have been involved with projects utilising TraDIS in E. coli, Salmonella, C. difficile, Bordetella, Pseudomonas and Staphylococcus.
In 2016, I moved to the University of East Anglia (UEA) to study the genetics of the non-aureus staphylococci, in particular relating to their biofilm forming ability. I also undertook a large-scale screen to identify antibiotic resistance determinants across a collection of >400 non-aureus strains before taking up this position at the Quadram Institute in late 2018.
Key Publications
Djeghout B, Saha S, Sajib MSI, Tanmoy AM, Islam M, Kay GL, Langridge GC, Endtz H, Wain J and Saha S (2018). Ceftriaxone-resistant Salmonella Typhi carries an IncI1-ST31 plasmid encoding CTX-M-15. J Med Micro 67: 620-627. 2. https://doi.org/10.1099/jmm.0.000727
Langridge GC, et al (2015). Patterns of genome evolution that have accompanied host adaptation in Salmonella. Proc Natl Acad Sci 112 (3): 863-868. 3. https://doi.org/10.1073/pnas.1416707112
Barquist L, Langridge GC, et al (2013). A comparison of dense transposon insertion libraries in the Salmonella serovars Typhi and Typhimurium. Nucl Acid Res 41 (8): 4549-4564. 4. https://doi.org/10.1093/nar/gkt148
Fookes M, Schroeder GN, Langridge GC, et al (2011). Salmonella bongori provides insights into the evolution of the salmonellae. PLoS Pathog 7(8): e1002191. 5. https://doi.org/10.1371/journal.ppat.1002191
Langridge GC et al (2009). Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants. Genome Res 19: 2308-2316. https://doi.org/10.1101/gr.097097.109
Related Case Studies
The genetics behind drug resistant enteric fever
