Currently researching into Staphylococcus AMR and TraDIS as well as carrying out evolutionary experiments into Salmonella and stresses involved in the food chain. My PhD at UEA was focused on Biochemistry, Biotechnology and Molecular Microbiology techniques. Over the years I have not only been a research scientist, a chef, a barrista, a lecturer are just a few of the other jobs that come to mind, but being in the lab is my calling and I always tend to come back to it.
Techniques I have picked up along the years involve human tissue processing, qRT-PCR, TaqMan, MIC, biofilm growth, continuous cultures (bacterial and colon model), protein purification, enzyme kinetic assays, HPLC, GC and a perfect filter coffee
The impact of copper, nitrate and carbon status on the emission of nitrous oxide by two species of bacteria with biochemically distinct denitrification pathways.
Felgate H, Giannopoulos G, Sullivan MJ, Gates AJ, Clarke TA, Baggs E, Rowley G, Richardson DJ.
Environ Microbiol. 2012 Jul;14(7):1788-800. doi: 10.1111/j.1462-2920.2012.02789.x. Epub 2012 May 30.
Mitigating release of the potent greenhouse gas N(2)O from the nitrogen cycle – could enzymic regulation hold the key?
Richardson D, Felgate H, Watmough N, Thomson A, Baggs E.
Trends Biotechnol. 2009 Jul;27(7):388-97. doi: 10.1016/j.tibtech.2009.03.009. Epub 2009 Jun 3. Review.
Resolving the contributions of the membrane-bound and periplasmic nitrate reductase systems to nitric oxide and nitrous oxide production in Salmonella enterica serovar Typhimurium.
Rowley G, Hensen D, Felgate H, Arkenberg A, Appia-Ayme C, Prior K, Harrington C, Field SJ, Butt JN, Baggs E, Richardson DJ.
Biochem J. 2012 Jan 15;441(2):755-62. doi: 10.1042/BJ20110971.
Nitrous oxide production in soil isolates of nitrate-ammonifying bacteria.
Stremińska MA, Felgate H, Rowley G, Richardson DJ, Baggs EM.
Environ Microbiol Rep. 2012 Feb;4(1):66-71. doi: 10.1111/j.1758-2229.2011.00302.x. Epub 2011 Oct 31.