Mark Philo


Contact via email

Core Science Resources - methods development and innovation

I am principle operator of QI mass spectrometry, also liaising with John Innes facilities.  I undertake untargeted work for which I used a quadrupole – time of flight instrument also more of my time is spent using the pair of triple quadrupole instruments for targeted work.

I am involved with many groups within QIB to meet mass spectrometry needs.  Popular work involves bile acid profiling from the gut, faeces and liver. A complimentary lipid profiling technique is newly developed to run alongside these analyses.

I also work closely with the Food and Health phytochemicals group to profile anthocyanins and other natural compounds, including analysis of intervention studies looking for targeted metabolites in urine and plasma.

Within the analytical sciences group I am involved in the determination of food fraud with respect to meat speciation and cheese by peptide profiling. I regularly undertake ad-hoc analyses from other groups to analyses toxins in milk or profile baby formula compared to breast milk.

Working alongside our NMR facilities I regularly prepare analytical samples and oversee some of the maintenance of this instrument. I also undertake commercial work through IFR-Extra which normally involved development of a niche method to meet the client’s requirements.

Key Publications

Watson A. D., Gunning Y., Rigby N. M., Philo M., Kemsley E. K. (2015) Rapid Meat Authentication via MRM Mass Spectrometry of Myoglobin Peptides Analytical Chemistry  87 10315-10322

Blokker B. A., Maijo M., Echeandia M., Galduroz M., Patterson A. M., Ten A., Philo M., Schungel R., Gutierrez-de Juan V., Halilbasic E., Fuchs C., Le Gall G., Milkiewicz M., Milkiewicz P., Banales J. M., Rushbrook S. M., Mato J. M., Trauner M., Müller M., Martínez-Chantar M. L., Varela-Rey M., Beraza N.. (2018)

Fine-tuning of SIRT1 expression is essential to protect the liver from cholestatic liver disease.

Hepatology (Baltimore, Md.)

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Hollands W. J., Tapp H., Defernez M., Perez-Moral N., Winterbone M. S., Philo M., Lucey A. J., Kiely M. E., Kroon P. A.. (2018)

Lack of acute or chronic effects of epicatechin-rich and procyanidin-rich apple extracts on blood pressure and cardiometabolic biomarkers in adults with moderately elevated blood pressure: a randomized, placebo-controlled crossover trial.

The American journal of clinical nutrition, 108, 1006-1014

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Ostertag L. M., Philo M., Tapp H. S., Colquhoun I. J., Saha S., Duthie G. G., Kemsley E. K., de Roos B., Kroon P. A., Le Gall G.. (2017)

Acute consumption of flavan-3-ol-enriched dark chocolate affects human endogenous metabolism – a randomized controlled human interventional trial

Journal of Proteome Research, 16, 2516-2526

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Gunning Y., Defernez M., Watson A. D., Beadman N., Colquhoun I. J., Le Gall G., Williamson D., Davis A. P., Kemsley E. K., Philo M., Garwood H.. (2017)

Esterified 16-O-methylcafestol is present in ground roast Arabica coffees: implications for authenticity testing

Food Chemistry, 248, 52-60

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Gunning Y., Watson A. D., Rigby N. M., Philo M., Peazer K., Kemsley E. K.. (2016)

Species determination and quantification in mixtures using MRM mass spectrometry of peptides applied to meat authentication

Journal of Visualized Experiments, 115, e54420

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Dower J. I., Geleijnse J. M., Kroon P. A., Philo M., Mensink M., Kromhout D., Hollman P. C. H.. (2016)

Does epicatechin contribute to the acute vascular function effects of dark chocolate? A randomized, crossover study.

Molecular Nutrition and Food Research, 60, 2379-2386

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