Dr Shikha Saha

Researcher

Optimising nutrient release from plant-based foods

View Cathrina Edwards's group

Biography

I have extensive knowledge of analytical chemistry, especially chromatography (reversed phase, normal phase, chiral phase, mixed mode, and ion-exchange), spectroscopy (UV, fluorescence, IR, and NMR), and liquid chromatography–mass spectrometry (LC-MS and LC-MS/MS) techniques.

My work as a senior analytical scientist at the Food & Health Programme involves sample preparation, identification of unknown compounds in different matrices, protein-specific peptide identification, and quantitative analysis of wide range of phytochemicals in plant or biological fluids of human and animal origins.

I am responsible for the development of novel analytical methods for the analysis of plant, food, microbial and human metabolites.

I am responsible for training and supervising post-graduate students, visiting scientists and post-docs that are using HPLC, LC-MS and LC-MS/MS techniques as part of their research projects.

I liaise extensively with external contractors regarding all aspects of equipment maintenance, and with obtaining information regarding procurement of new instrumentation.

Day-Walsh P,Shehata E,Saha S,Savva GM,Nemeckova B,Speranza J,Kellingray L,Narbad A,Kroon PA. (2021)

The use of an in-vitro batch fermentation (human colon) model for investigating mechanisms of TMA production from choline, L-carnitine and related precursors by the human gut microbiota.

European journal of nutrition

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Huynh T, Bowater R, Bernuzzi F, Saha S, Wormstone M. (2021)

GSH Levels Serve As A Biological Redox Switch Regulating Sulforaphane Induced Cell Fate in Human Lens Cells

Investigative ophthalmology and visual science

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Saha S, Day-Walsh P, Shehata E, KROON PA. (2021)

Development and Validation of a LC-MS/MS Technique for the Analysis of Short Chain Fatty Acids in Tissues and Biological Fluids without Derivatisation Using Isotope Labelled Internal Standards

Molecules, 10.3390/molecules26216444

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Neequaye M, Stavnstrup S, Harwood W, Lawrenson T, Hundleby P, Irwin J, Troncoso-Rey P, Saha S, Traka MH, Mithen R, Østergaard L. (2021)

CRISPR-Cas9-Mediated Gene Editing of MYB28 Genes Impair Glucoraphanin Accumulation of Brassica oleracea in the Field.

The CRISPR journal, 4, 416-426

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Mulet-Cabero A. I., Torcello-Gómez A., Saha S., Mackie A. R., Wilde P. J., Brodkorb A.. (2020)

Impact of caseins and whey proteins ratio and lipid content on in vitro digestion and ex vivo absorption.

Food Chemistry, 319, 126514

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Diaz M., Sayavedra L., Atter A., Mayer M. J., Amoa-Awua W., Narbad A., Saha S.. (2020)

Lactobacillus garii sp. nov., isolated from a fermented cassava product.

International journal of systematic and evolutionary microbiology

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Traka M., Melchini A., Coode-Bate J., Al-Kadhi O., Shikha S., Defernez M., Troncoso Rey P., Kibblewhite H., O'Neill C., Bernuzzi F., Mythen L., Hughes J., Needs P., Dainty J., Savva G., Mills R., Ball R., Cooper C., Mithen R.. (2019)

Transcriptional changes in prostate of men on active surveillance following a 12-month glucoraphanin-rich broccoli intervention – results from the ESCAPE randomized controlled trial

AJCN

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Sivapalan T., Melchini A., Coode-Bate J., Needs P., Mithen R., Saha S.. (2019)

LC-MS/MS method for measuring S-methyl-L-cysteine andS-methyl-L-cysteine sulfoxide in human specimens by using isotope labelledinternal standards

Molecules, 24, 2427

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