Scientific Publications
Development of a multiplex real-time PCR method for simultaneous detection of Vibrio parahaemolyticus, Listeria monocytogenes and Salmonella spp. in raw shrimp
Food Control, 51, 31-36
Vibrio parahaemolyticus , Listeria monocytogenes and Salmonella spp. are important pathogens contaminating seafood in China. In this study, we developed an ef fi cient multiplex real-time PCR for the simultaneous detection of V. parahaemolyticus, L. monocytogenes and Salmonella spp. in raw shrimp without a prior enrichment step. In a test using 28 target and non-target strains only the targets were detected and two calibration curves, for pure cultures and arti fi cially contaminated samples, were used to evaluate the ef ficiencies of this method. Amplification effi ciencies of this multiplex real-time PCR were excellent in pure cultures and arti ficially contaminated shrimps. The limits of detection in artifi cially contaminated shrimps were 112 CFU/g for V. parahaemolyticus, 158 CFU/g for L. monocytogenes and 103 CFU/g for Salmonella spp. We validated this multiplex real-time PCR method on 48 commercial samples and the results were comparable to standard culture methods. This ef fi cient multiplex real-time PCR, where each test takes only 50 min after DNA extraction, is a useful tool for high-throughput surveillance of V. parahaemolyticus, L. monocytogenes and Salmonella spp. in seafood products
Food Control, 51, 31-36
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