Scientific Publications
In situ mapping of the effect of additional mutations on starch granule structure in amylose extender (ae) maize kernels
Carbohydrate Polymers, 118, 199-208
Optical (KI/I2-staining, polarised) and FTIR microscopy has been used to monitor starch granule structurewithin wild-type (wt), GEMS-0067 and waxy-amylose-extender (wx-ae) maize mutant kernels. In theGEMS-0067 mutant containing the high amylose modifier (HAM) gene(s) plus the recessive ae gene,structural heterogeneity characteristic of the ae mutation was reduced markedly. However, enhancedvariation in granule shape and size was observed distributed spatially within the kernel, which appearsto be related to new heterogeneity in internal starch granule structure. In wx-ae starch mutants the aegene led to heterogeneity of starch granule structure equivalent to that in single ae mutants, plus newstructural heterogeneity coincident with novel induced variation in granule size and shape.
Carbohydrate Polymers, 118, 199-208
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