Scientific Publications
Rapid Meat Authentication via MRM Mass Spectrometry of Myoglobin Peptides
Analytical Chemistry, 87, 10315-10322
A rapid MRM mass spectrometry method for the detection and relative quantitation of the adulteration of meat with that of an undeclared species is presented. Our approach uses corresponding proteins from the different species under investigation, and corresponding peptides from those proteins, or CPCP. Selected peptide markers can be used for species detection. The use of ratios of MRM transition peak areas for corresponding peptides is proposed for relative quantitation. The approach is introduced using myoglobin from 4 meats: beef, pork, horse and lamb. Focusing in the present work on species identification, using predictive tools we determine peptide markers that allow the identification of all four meats, and detection of one meat added to another at levels of less than 1% w/w. Candidate corresponding peptide pairs to be used for the relative quantification of one meat added to another have been observed. Preliminary quantitation data presented here are encouraging.
Analytical Chemistry, 87, 10315-10322
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