Reply to: Microbial dark matter could add uncertainties to metagenomic trait estimations.
Nature microbiology
In a recent paper, we used soil metagenomes from global biomes to assess life history strategies across soil bacterial communities1. We compiled 102 traits in a multi-table coinertia analysis to characterize the life history strategies of soil bacteria. Although we only used annotated reads for the calculation of most traits, full metagenomes were used to quantify bacterial average genome size (AGS) and ribosomal RNA operon copy numbers.
Nature microbiology
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