Scientific Publications
Stability of sunflower 2S albumins and LTP to physiologically relevant in vitro gastrointestinal digestion
Food Chemistry, 138, 2374-2381
In order for a protein to elicit a systemic allergic response it must reach the circulatory system throughthe intestinal mucosa as a sufficiently large fragment with adequate structural integrity. Sunflower LTPand 2S albumins (SFA8 and three mixed fractions of Alb1 and Alb2) were digested in simulated gastricfluid (SGF) for 2 h and the conditions were then changed to mimic the intestinal environment for a further2 h digestion. The effects of phosphatidylcholine (PC) and emulsification on the digestibility of theproteins were investigated. PC protected all of the proteins studied against both gastric and intestinaldigestive enzymes but to different extents. Emulsification of SFA8 resulted in strong protection againstdigestion, which was further enhanced by the presence of PC in the SGF. These results highlight theimportance of considering real food structures such as emulsified systems and also the gastrointestinalenvironment that proteins are exposed to once consumed when assessing allergenicity.
Food Chemistry, 138, 2374-2381
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