Trafficking and processing of bacterial proteins by mammalian cells: Insights from chondroitinase ABC.

Muir E., Raza M., Ellis C., Burnside E., Love F., Heller S., Elliot M., Daniell E., Dasgupta D., Alves N., Day P., Fawcett J., Keynes R.. (2017)

PLoS ONE, 12, e0186759

There is very little reported in the literature about the relationship between modifications of bacterial proteins and their secretion by mammalian cells that synthesize them. We previously reported that the secretion of the bacterial enzyme Chondroitinase ABC by mammalian cells requires the strategic removal of at least three N-glycosylation sites. The aim of this study was to determine if it is possible to enhance the efficacy of the enzyme as a treatment for spinal cord injury by increasing the quantity of enzyme secreted or by altering its cellular location.

PLoS ONE, 12, e0186759

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