Use of metabolomics and fluorescence recoveryafter photobleaching to study the bioavailabilityand intestinal mucus diffusion of polyphenolsfrom cauliflower waste

Gonzales G. B., Smagghe G., Mackie A. R., Grootaert C., Bajka B. H., Rigby N. M., Raes C., van Kamp J.. (2015)

Journal of Functional Foods, 16, 403413

The analysis of the bioaccessibility and bioavailability of polyphenols from cauliflower wastewas achieved using targeted metabolomics and chemometric approaches. Changes in phenolicprofile throughout the in vitro digestion and Caco-2 transport were investigated usingLC-MS combined with principal components analysis and orthogonal partial least squares–discriminant analysis, and diffusion of polyphenols through intestinal mucus was monitoredusing fluorescence recovery after photobleaching (FRAPb). Results showed that recovery ofpolyphenols in the gastric phase was approximately 76–106% whilst losses of up to 70% wereobserved after the intestinal phase. Kaempferol-3-O-diglucoside and kaempferol-3-Odiglucoside-7-O-glucoside were found to permeate intact through the Caco-2 cells in verysmall amounts (0.3% recovery). Polyphenols also diffused rapidly through intestinal mucuswithout altering their biophysical properties.We conclude that targeted metabolomics andFRAPb are rapid and convenient tools to study the recovery of polyphenols during in vitrodigestion, mucosal diffusion and Caco-2 transport.

Journal of Functional Foods, 16, 403413

View Publication