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3. Sample Collection and Initial Processing of Faecal Samples

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Collection and Initial Processing of Samples

Sarah Phillips, Thomas Atkinson, Rachel Watt

This protocol discusses the impact that different sample collection methods have on microbiome research and provides examples of methods that have been used at the Quadram Institute for these purposes. It also outlines the initial processing of samples on receipt.

3. Collection and Initial Processing of Samples v1.0[pdf]

Creative Commons License
Quadram Institute Best Practice in Microbiome Research: Collection and Initial Processing of Faecal Samples by Sarah Phillips, Thomas Atkinson, Rachel Watt is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

    • Introduction
    • 1. Study Design Considerations
    • 2. Sample Size for a Microbiome Experiment
    • 3. Collection & Processing of Faecal Samples
    • 4. DNA Extraction and Quality Control
    • 5. 16S Illumina Library Preparation
    • 6. Shotgun Metagenomic Illumina Library Preparation
    • 7. Microbiome Informatics
    • 8. Statistical Analysis of Microbiome Data
    • 9. Viromics-Specific Protocols
    • 10. Mycobiome-Specific Protocols
    • About the Quadram Institute
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    Quadram Institute

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